Biophysical Chemistry of Proteins An Introduction to Laboratory Methods /
This textbook, designed for all scientists interested in protein research, provides a thorough overview of laboratory methods for the biophysical chemistry of proteins. This new edition, completely restructured and expanded for ease of learning, includes sections on analytical techniques, working wi...
Tallennettuna:
| Päätekijä: | |
|---|---|
| Yhteisötekijä: | |
| Aineistotyyppi: | Elektroninen E-kirja |
| Kieli: | englanti |
| Julkaistu: |
New York, NY :
Springer US : Imprint: Springer,
2025.
|
| Painos: | 2nd ed. 2025. |
| Aiheet: | |
| Linkit: | https://doi.org/10.1007/978-1-0716-4124-8 |
| Tagit: |
Lisää tagi
Ei tageja, Lisää ensimmäinen tagi!
|
Sisällysluettelo:
- Analytical techniques
- Microscopy
- Single molecule techniques
- Preparation of cells and tissues for microscopy
- Principles of optical spectroscopy
- Photometry
- Fluorimetry
- Chemiluminescence
- Electrophoresis
- Immunological methods
- Isotope techniques
- Purification of proteins
- Homogenisation and fractionisation of cells and tissues
- Isolation of organelles
- Precipitation methods
- Chromatography
- Membrane proteins
- Determination of protein concentration
- Cell culture
- Protein modification and inactivation
- General technical remarks
- Amine-reactive reagents
- Thiol- and disulphide reactive reagents
- Reagents for other groups
- Cross-linkers
- Detection methods
- Spontaneous reactions in proteins
- Protein size and shape
- Centrifugation
- Osmotic pressure
- Diffusion
- Viscosity
- Non-resonant interactions with electromagnetic waves
- Protein structure
- Protein sequencing
- Synthesis of peptides
- Protein secondary structure
- Structure of protein-ligand complexes
- 3D-structures
- Folding and unfolding of proteins
- Enzyme kinetics
- Steady-state kinetics
- Leaving the steady state: Analysis of progress curves
- Reaction velocities
- Isotope effects
- Isotope exchange
- Protein-ligand interactions
- General conditions for interpretable results
- Binding equations
- Methods to measure binding equilibria
- Temperature effects on binding equilibrium and reaction rate
- Industrial enzymology
- Industrial enzyme use
- Immobilised enzymes
- Special statistics
- Quality control
- Testing whether or not a model fits the data
- Appendix
- List of symbols
- Greek alphabet
- Properties of electrophoretic buffers
- Bond properties
- Acronyms.