Cryo-Electron Tomography Structural Biology in situ /
This book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facili...
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Cham :
Springer International Publishing : Imprint: Springer,
2024.
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| Edition: | 1st ed. 2024. |
| Series: | Focus on Structural Biology,
11 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-3-031-51171-4 |
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| 245 | 1 | 0 | |a Cryo-Electron Tomography |h [electronic resource] : |b Structural Biology in situ / |c edited by Friedrich Förster, Ariane Briegel. |
| 250 | |a 1st ed. 2024. | ||
| 264 | 1 | |a Cham : |b Springer International Publishing : |b Imprint: Springer, |c 2024. | |
| 300 | |a 1 online resource (VIII, 389 p. 92 illus.). |b 78 illus. in color. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 341 | 0 | |b Table of contents navigation |2 onix | |
| 341 | 0 | |b Single logical reading order |2 onix | |
| 341 | 0 | |b Short alternative textual descriptions |2 onix | |
| 341 | 0 | |b Use of color is not sole means of conveying information |2 onix | |
| 341 | 0 | |b Use of high contrast between text and background color |2 onix | |
| 341 | 0 | |b Next / Previous structural navigation |2 onix | |
| 341 | 0 | |b All non-decorative content supports reading without sight |2 onix | |
| 347 | |a text file |b PDF |2 rda | ||
| 490 | 1 | |a Focus on Structural Biology, |x 2542-9566 ; |v 11 | |
| 505 | 0 | |a 1. Fundamentals of Instrumentation and electron optics For cryo-electron tomography -- 2. Principles of tomographic reconstruction -- 3. Cryo-preparation for cellular tomography -- 4. Cryo-FIB milling -- 5. In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques -- 6. Labeling approaches and correlation with light microscopy (Cryo) CLEM -- 7. Data collection -- 8. Subvolume averaging and classification -- 9. High resolution tomography/ Molecular model building and visualization -- 10. Beam-induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography -- 11. Tomogram segmentation -- 12. Electron tomography of cryo-fixed and resin-embedded samples -- 13. Democratization of tomographic data / FAIR data. | |
| 520 | |a This book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that are preserved in a near-native state, at (macro)-molecular resolution and in three dimensions. Thus, this technique is a unique tool to gain insights into how biomolecules collaborate in orchestrating fundamental biological processes, how mutations cause diseases, pathogens cause infections, and to develop novel therapeutics to treat such illnesses. This book provides a unique reference for the emerging field of cryo-ET. The topics covered range from the fundamental principles of imaging to sample preparation, data analysis, and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, and molecular biology and highly valuable for the more experienced and specialized PhD student. Furthermore, it stands as a state-of-the–art source of knowledge for the established senior scientist within the field of structural biology. | ||
| 532 | 8 | |a Accessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. | |
| 532 | 8 | |a No reading system accessibility options actively disabled | |
| 532 | 8 | |a Publisher contact for further accessibility information: accessibilitysupport@springernature.com | |
| 588 | |a Print version record. | ||
| 650 | 0 | |a Biomolecules. | |
| 650 | 0 | |a Physical biochemistry. | |
| 650 | 0 | |a Macromolecules. | |
| 650 | 0 | |a Biology |x Technique. | |
| 650 | 0 | |a Biophysics. | |
| 650 | 0 | |a Immunology. | |
| 650 | 0 | |a Materials |x Microscopy. | |
| 650 | 1 | 4 | |a Structural Biology. |
| 650 | 2 | 4 | |a Biophysical Methods. |
| 650 | 2 | 4 | |a Immunology. |
| 650 | 2 | 4 | |a Microscopy. |
| 700 | 1 | |a Förster, Friedrich. |e editor. |0 (orcid)0000-0002-6044-2746 |1 https://orcid.org/0000-0002-6044-2746 |4 edt |4 http://id.loc.gov/vocabulary/relators/edt | |
| 700 | 1 | |a Briegel, Ariane. |e editor. |0 (orcid)0000-0003-3733-3725 |1 https://orcid.org/0000-0003-3733-3725 |4 edt |4 http://id.loc.gov/vocabulary/relators/edt | |
| 710 | 2 | |a SpringerLink (Online service) | |
| 773 | 0 | |t Springer Nature eBook | |
| 776 | 0 | 8 | |i Printed edition: |z 9783031511707 |
| 776 | 0 | 8 | |i Printed edition: |z 9783031511721 |
| 776 | 0 | 8 | |i Printed edition: |z 9783031511738 |
| 830 | 0 | |a Focus on Structural Biology, |x 2542-9566 ; |v 11 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-3-031-51171-4 |
| 912 | |a ZDB-2-SBL | ||
| 912 | |a ZDB-2-SXB | ||
| 950 | |a Biomedical and Life Sciences (SpringerNature-11642) | ||
| 950 | |a Biomedical and Life Sciences (R0) (SpringerNature-43708) | ||