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Cryo-Electron Tomography Structural Biology in situ /

This book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facili...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Förster, Friedrich (Editor), Briegel, Ariane (Editor)
Format: Electronic eBook
Language:English
Published: Cham : Springer International Publishing : Imprint: Springer, 2024.
Edition:1st ed. 2024.
Series:Focus on Structural Biology, 11
Subjects:
Biomolecules.
Physical biochemistry.
Macromolecules.
Biology > Technique.
Biophysics.
Immunology.
Materials > Microscopy.
Structural Biology.
Biophysical Methods.
Microscopy.
Online Access:https://doi.org/10.1007/978-3-031-51171-4
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Table of Contents:
  • 1. Fundamentals of Instrumentation and electron optics For cryo-electron tomography
  • 2. Principles of tomographic reconstruction
  • 3. Cryo-preparation for cellular tomography
  • 4. Cryo-FIB milling
  • 5. In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques
  • 6. Labeling approaches and correlation with light microscopy (Cryo) CLEM
  • 7. Data collection
  • 8. Subvolume averaging and classification
  • 9. High resolution tomography/ Molecular model building and visualization
  • 10. Beam-induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography
  • 11. Tomogram segmentation
  • 12. Electron tomography of cryo-fixed and resin-embedded samples
  • 13. Democratization of tomographic data / FAIR data.

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