Single-Cell Omics in Microbiome Research Exploring Microbial Diversity Across Ecosystems /

This volume introduces the revolutionary field of microbial single-cell omics, a suite of cutting-edge techniques that uncover the genetic, functional, and metabolic signatures of individual microbial cells. From the discovery of new microbial lineages to decoding virus-host interactions, this book...

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Bibliographische Detailangaben
Körperschaft: SpringerLink (Online service)
Weitere Verfasser: Rinke, Christian (HerausgeberIn), Džunková, Mária (HerausgeberIn)
Format: Elektronisch E-Book
Sprache:Englisch
Veröffentlicht: Cham : Springer Nature Switzerland : Imprint: Springer, 2025.
Ausgabe:1st ed. 2025.
Schriftenreihe:The Microbiomes of Humans, Animals, Plants, and the Environment, 7
Schlagworte:
Online-Zugang:https://doi.org/10.1007/978-3-032-07527-7
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490 1 |a The Microbiomes of Humans, Animals, Plants, and the Environment,  |x 2662-6128 ;  |v 7 
505 0 |a Chapter 1: A Hitchhikers Guide to Microbial Single-Cell Omics -- Chapter 2: Overview of laboratory workflows for microbial single-cell genomics -- Chapter 3: Characterization of microbial diversity and mobile genetics elements through single-cell genomics -- Chapter 4: FISH-FACS enabled targeted recovery of genomes from uncultivated environmental microbial populations -- Chapter 5: Advancements in the application of activity-based studies for separation of microbial populations via fluorescence-activated cell sorting -- Chapter 6: Technical advancements in microbial single-cell omics analysis -- Chapter 7: Challenges and Future Perspectives for Single-Cell Multi-Omics in Prokaryotes -- Chapter 8: Single-Cell Sorting to Assess Phage Sensitivity in Heterogeneous Bacterial Populations. 
520 |a This volume introduces the revolutionary field of microbial single-cell omics, a suite of cutting-edge techniques that uncover the genetic, functional, and metabolic signatures of individual microbial cells. From the discovery of new microbial lineages to decoding virus-host interactions, this book highlights the transformative impact of single-cell genomics, transcriptomics, proteomics, and metabolomics. With expert protocols and innovative workflows, the book provides practical guidance on fluorescence-activated cell sorting (FACS), microfluidic encapsulation, and advanced genome amplification techniques. It also explores targeted methods, like FISH-FACS for recovering rare microbes, activity-based cell separation, and multi-omics approaches that integrate genomic, transcriptomic, and proteomic data. The authors discuss future developments and the immense benefits of analysing and understanding microbes one cell at a time, offering a glimpse into how this knowledge can transform microbial ecology, biomedicine, and biotechnology. The book is aimed at researchers and students of environmental microbiology who wish to expand their knowledge of this ground-breaking approach and the underlying technology. 
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650 0 |a Microbial ecology. 
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650 0 |a Microbial genetics. 
650 1 4 |a Environmental Microbiology. 
650 2 4 |a Microbiology Techniques. 
650 2 4 |a Microbial Genetics. 
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