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Protocols for Studying Pathogenic Fungi

This book is a compilation of protocols for conducting experiments with a variety of yeasts, including S. cerevisiae, Candida albicans, non-albicans Candida sp., Cryptococcus sp.; and molds, including Aspergillus sp., Fusarium sp., Mucor, Rhizopus. The chapters not only discuss National Committee fo...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijät: Pasrija, Ritu (Tekijä), Kumari, Deepika (Tekijä)
Yhteisötekijä: SpringerLink (Online service)
Aineistotyyppi: Elektroninen E-kirja
Kieli:englanti
Julkaistu: Singapore : Springer Nature Singapore : Imprint: Springer, 2025.
Painos:1st ed. 2025.
Aiheet:
Fungi.
Mycology.
Microbiology.
Biofilms.
Microbial genetics.
Microbial Genetics.
Linkit:https://doi.org/10.1007/978-981-96-7047-5
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Sisällysluettelo:
  • 1. Introduction to BSL-2 Facilities
  • 2. Handling, Culturing, Preservation and Revival of Fungal Pathogens
  • 3. Counting Colony Forming Units (CFU) to Estimate Viable Fungal Cells in a Sample
  • 4. Growth Curve Preparation for the Calculation of Fungal Cell Count Over Time
  • 5. Microtiter Assay for Drug Susceptibility Testing of Pathogenic Fungi in Broth
  • 6. Disk Diffusion Assay for Testing the Drug Sensitivity of Pathogenic Fungi in Agar
  • 7. Spot Assay for Comparing Drug Susceptibility of Pathogenic Fungi in Agar
  • 8. Time-Kill Kinetics to Determine the Number of Living Cells Over a Period of Time
  • 9. Checkerboard Analysis to Calculate the Fractional Inhibitory Concentration (FIC) Index of Pathogenic Fungi for Drug Interaction
  • 10. Hyphal Induction in Yeasts and Molds
  • 11. Isolation of Genomic DNA from Pathogenic Fungi
  • 12. Exopolysaccharide Secretion by Pathogenic Fungi
  • 13. Biofilm Formation Test and Determination of the Effect of Drugs on their Formation
  • 14. Induction of Capsule Formation in Cryptococcus sp
  • 15. Melanin Formation in Cryptococcus sp
  • 16. Urease Assay for Cryptococcus sp
  • 17. Phospholipase Assay for the Detection of Virulence of Pathogenic Fungi
  • 18. Hydrophobicity Test for Virulence of Pathogenic Fungi
  • 19. Adhesion of Fungal Cells to Buccal Epithelial Cells
  • 20. Sterols Extraction, Analysis and Quantification in Pathogenic Fungi
  • 21. Assessing Mitochondrial Fitness by Assessing Microbial Growth on Non-Fermentable Carbon Sources
  • 22. In Vivo Assay using Caenorhabditis elegans.

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