Techniques for biochemical analysis /
This book, authored by Khursheed Hussain, Sameena Maqbool Lone, Khalid Zaffar Masoodi, and Syed Minu Balkhi, provides a comprehensive guide to biochemical analysis techniques. It covers various laboratory procedures, preparation of solutions and buffers, qualitative analysis of carbohydrates and pro...
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| Altres autors: | |
|---|---|
| Format: | eBook |
| Idioma: | anglès |
| Publicat: |
[S.l.] :
Academic Press,
2024.
|
| Matèries: | |
| Accés en línia: | ScienceDirect |
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Taula de continguts:
- Front Cover
- Techniques for Biochemical Analysis
- Copyright Page
- Contents
- Preface
- 1 Introduction to biochemistry
- Historical background
- Various branches of biochemistry
- Molecular biology
- Cell biology
- Metabolism
- Genetics
- Other branches include
- Importance of biochemistry
- 2 Introduction to laboratory procedures
- Instructions to work in the laboratory
- Dos and don'ts in the laboratory
- Dos
- Don'ts
- Laboratory equipment care
- Cleaning
- Calibration
- Repairs and refurbishments
- Quality replacement
- 3 Preparation of solutions and buffers
- Common definitions
- Preparation of solutions
- Percent solutions
- Molarity (M)
- Normality (N)
- Molality (M)
- Parts per million (ppm)
- Preparation of buffers
- Characteristics of a buffer solution
- Types of buffer solutions
- Mechanism of buffering action
- Acidic buffer
- Basic buffer
- Henderson's equation (pH of buffer)
- Acidic buffer
- Basic buffer
- Buffering capacity
- Applications of buffer in chemistry
- 4 Qualitative analysis of carbohydrates
- Classification of carbohydrates
- Monosaccharides
- Glucose
- Fructose
- Galactose
- Disaccharides
- Maltose
- Lactose
- Sucrose
- Trehalose
- Trisaccharides
- Polysaccharides
- Tests for carbohydrates
- Physical tests
- Chemical tests
- Molisch test
- Rationale
- Reagents required (Molisch reagent)
- Protocol
- Observation
- Inference
- Safety considerations and standards
- Iodine test
- Rationale
- Reagents required (iodine's reagent)
- Protocol
- Observation
- Inference
- Safety considerations and standards
- Benedict's test (reduction under alkaline condition)
- Rationale
- Reagents required (Benedict's qualitative reagent)
- Protocol
- Observation
- Inference
- Safety considerations and standards
- Barfoed's test (reduction under acidic medium).
- Rationale
- Reagents required (Barfoed's reagent)
- Protocol
- Observation
- Inference
- Safety considerations and standards
- 5 Qualitative analysis of proteins
- Classification of carbohydrates
- Functions of proteins
- Chemical tests for the estimation proteins
- Biuret test
- Rationale
- Reagents required (Biuret's reagent)
- Protocol
- Observation
- Inference
- Safety considerations and standards
- Ninhydrin test
- Rationale
- Reagents required (Ninhydrin's reagent)
- Protocol
- Observation
- Inference
- Safety considerations and standards
- Xanthoproteic test
- Rationale
- Reagents required
- Protocol
- Observation
- Inference
- Safety considerations and standards
- Further reading
- 6 pH (negative logarithm of hydrogen ion concentration)
- Measurement of pH
- pH indicators
- Measurement of pH
- 7 Spectrophotometry
- Rationale
- Qualitative photometric assays
- Quantitative photometric assays
- Standard curve
- Measurement of absorbance
- Colorimeter
- Spectrophotometer
- Ultraviolet and infrared spectra
- Fluorimetry
- Application of spectrophotometry
- 8 Estimation of carbohydrates
- Sample extraction for estimation of sugars
- Reagents required
- Protocol
- Safety considerations and standards
- Estimation of total sugars by anthrone method
- Rationale
- Reagents required
- Preparation of the standard curve of glucose
- Protocol
- Analysis and statistics
- Calculation
- Safety considerations and standards
- Estimation of total sugars by phenolsulfuric method
- Rationale
- Reagents required
- Protocol
- Analysis and statistics
- Calculation
- Estimation of reducing sugars by NelsonSomogyi method
- Rationale
- Reagents required
- Preparation of the standard curve of glucose
- Protocol
- Analysis and statistics
- Calculation
- Safety considerations and standards.
- Reagents required
- Protocol
- Enzyme extraction process
- Enzyme assay procedure
- Analysis and statistics
- Determination of lactate dehydrogenase activity
- Rationale
- Reagents required
- Protocol
- Enzyme extraction process
- Enzyme assay procedure
- Analysis and statistics
- Determination of chitinase activity
- Rationale
- Reagents required
- Protocol
- Enzyme extraction process
- Enzyme assay procedure
- Analysis and statistics
- Determination of polygalacturonase activity
- Rationale
- Reagents required
- Protocol
- Enzyme extraction process
- Enzyme assay procedure
- Analysis and statistics
- Determination of pectin methyl esterase activity
- Rationale
- Reagents required
- Protocol
- Enzyme extraction process
- Enzyme assay procedure
- Analysis and statistics
- Determination of l-phenylalanine ammonia lyase activity
- Rationale
- Reagents required
- Protocol
- Enzyme extraction process
- Enzyme assay procedure
- Analysis and statistics
- Determination of tyrosine ammonia lyase activity
- Rationale
- Reagents required
- Protocol
- Enzyme extraction process
- Enzyme assay procedure
- Determination of polyphenol oxidase or DOPA oxidase activity
- Rationale
- Reagents required
- Protocol
- Enzyme extraction process
- Enzyme assay procedure
- Analysis and statistics
- Determination of phytase activity
- Rationale
- Reagents required
- Protocol
- Enzyme extraction process
- Enzyme assay procedure
- Analysis and statistics
- Determination of malate dehydrogenase activity
- Rationale
- Reagents required
- Protocol
- Enzyme extraction process
- Enzyme assay procedure
- Analysis and statistics
- Determination of indoleacetic acid oxidase activity
- Rationale
- Reagents required
- Protocol
- Analysis and statistics
- Determination of ascorbic acid oxidase activity
- Rationale.