Livestock genome editing tools /
Livestock Genome Editing Tools introduces applications and improvements to a series of new genome editing techniques in livestock, such as pigs, cattle, and sheep.These tools include zinc finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN), and the clustered regularly int...
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| Formato: | eBook |
| Lenguaje: | inglés |
| Publicado: |
London :
Academic Press,
2024.
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| Materias: | |
| Acceso en línea: | ScienceDirect |
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| 001 | on1430500615 | ||
| 003 | OCoLC | ||
| 005 | 20260915095206.0 | ||
| 006 | m o d | ||
| 007 | cr |n||||||||| | ||
| 008 | 240420s2024 enk o 000 0 eng d | ||
| 040 | |a YDX |b eng |c YDX |d OPELS |d OCLCO |d N$T |d UKKRT |d OCLCQ |d OCLCL |d SFB |d OCLCF |d OCLCO | ||
| 020 | |a 9780128191002 |q (electronic bk.) | ||
| 020 | |a 0128191007 |q (electronic bk.) | ||
| 020 | |z 9780128190999 | ||
| 035 | |a (OCoLC)1430500615 | ||
| 050 | 4 | |a SF756.5 | |
| 082 | 0 | 4 | |a 636.08/21 |2 23/eng/20240506 |1 https://id.oclc.org/worldcat/ddc/E3yxVwVjmJDbQM8gXmW4wmgjQG |
| 245 | 0 | 0 | |a Livestock genome editing tools / |c editor-in-chief: Kui Li. |
| 260 | |a London : |b Academic Press, |c 2024. | ||
| 300 | |a 1 online resource | ||
| 505 | 8 | |a 2.3.8.1 Applications of CRISPR/Cas9 genome editing -- 2.3.9 Applications of gene expression regulation -- 2.3.10 Prospects -- 2.4 Base editing -- 2.4.1 DNA editing technologies -- 2.4.1.1 Cytosine base editor technology -- 2.4.1.2 Adenine-based editor technology -- 2.4.1.3 RNA editing technology -- 2.4.1.4 Applications of single-base editing technology -- 2.4.2 Summary and prospects of single-base editing technology -- References -- 3 Associated technologies for genome editing -- 3.1 Pronuclear microinjection -- 3.1.1 Generation of genetically engineered pigs using pronuclear microinjection technology -- 3.1.1.1 Collection and culture of pronuclear-stage embryos of pigs -- 3.1.1.1.1 Collection of pronuclear-stage embryos of pigs fertilized under in vivo conditions -- Preparation of donor sows -- Superovulation and fertilization of donor sows -- Recovery of pronuclear-stage embryos -- Selection of pronuclear-stage embryos -- 3.1.1.1.2 In vitro maturation of porcine oocytes and production of pronuclear-stage embryos fertilized under in vitro condi... -- In vitro maturation of porcine oocytes -- Treatment of mature porcine oocytes -- Pretreatment of porcine sperm and in vitro fertilization -- 3.1.1.1.3 In vitro maturation of porcine oocytes and production of pronuclear-stage embryos by intracytoplasmic sperm injection -- Pretreatment of sperm -- Preparation of the intracytoplasmic sperm injection operation dish -- Intracytoplasmic sperm injection process -- 3.1.1.2 Production and purification of the target gene fragments -- 3.1.1.3 Microinjection of zygotes -- 3.1.1.4 Preparation of recipient sows -- 3.1.1.5 Embryo transfer -- 3.1.1.6 Postoperative management of sows and identification of positive individuals -- 3.1.2 Research status of genetically engineered animal generation by pronuclear microinjection -- 3.2 Cytoplasmic injection. | |
| 505 | 8 | |a 3.2.1 Research progress on cytoplasmic injection technology -- 3.2.2 Mechanism for obtaining transgenes via cytoplasmic injection -- 3.2.3 Factors affecting cytoplasmic injection -- 3.2.3.1 Injection technique -- 3.2.3.2 Size of the zygotes -- 3.2.3.3 Structure and concentration of exogenous genes -- 3.2.3.4 Assisted coinjection of other components -- 3.2.3.5 Injection of a transposon system -- 3.2.4 Comparison of cytoplasmic and pronuclear microinjections -- 3.2.4.1 Survival rate of injected embryos -- 3.2.4.2 Transgenesis efficiency -- 3.2.4.3 Formation of chimeras -- 3.2.4.4 Costs -- 3.2.4.5 Application prospects for CI -- 3.3 Somatic cell nuclear transfer technology -- 3.3.1 Introduction of somatic cell nuclear transfer technology -- 3.3.2 The main methods and processes of somatic cell cloning -- 3.3.2.1 Preparation of nuclear donor cells -- 3.3.2.2 Oocyte collection and in vitro maturation -- 3.3.2.3 Enucleation of recipient oocytes -- 3.3.2.4 Nuclear transfer of donor cells -- 3.3.2.5 Fusion and activation of reconstructed embryos -- 3.3.2.6 In vitro culture and transplantation of reconstructed embryos -- 3.3.2.7 Care of sows during pregnancy and farrowing -- 3.3.3 Operation for pig embryo transfer -- 3.3.3.1 Selection of recipient sows -- 3.3.3.2 Induction of anesthesia and securing the pig -- 3.3.3.3 Maintenance of anesthesia and embryo transfer -- 3.3.3.4 Suturing the incision -- 3.3.3.5 Care of newborn piglets -- 3.3.3.5.1 Prevention of the birth of disadvantaged piglets -- 3.3.3.5.2 Attending and assisting during farrowing -- 3.3.3.5.3 First aid for dying piglets after birth -- 3.3.3.5.4 Colostrum intake of newborn piglets -- 3.3.3.5.5 Crossfostering of newborn piglets -- 3.3.3.5.6 Environmental requirements for newborn piglets -- 3.3.3.5.7 Timely iron supplementation for newborn piglets -- 3.3.3.5.8 Vaccination for piglets. | |
| 505 | 8 | |a 3.3.4 Present problems in somatic cell cloning technology -- 3.3.4.1 Low cloning efficiency and low survival rate of offspring -- 3.3.4.2 The abnormal development of the placenta -- 3.3.4.3 The source of mitochondria -- 3.3.4.4 The abnormal process of reprogramming -- 3.3.5 The prospects of somatic cell cloning technology -- 3.4 Electroporation -- 3.4.1 Model and mechanism of electroporation -- 3.4.2 Application of electroporation in vivo -- 3.4.3 Factors affecting the efficiency of electroporation -- 3.4.4 The problems and prospects of electroporation technology -- 3.5 RNA interference technology -- 3.5.1 The discovery of RNAi -- 3.5.2 The mechanism of RNAi -- 3.5.2.1 Initiation stage -- 3.5.2.2 Maintenance stage -- 3.5.2.3 Signal amplification stage -- 3.5.3 The biological characteristics of RNAi -- 3.5.4 Factors affecting the efficiency of RNAi -- 3.5.4.1 The construction of RNAi -- 3.5.4.2 The modification of RNAi sequences -- 3.5.4.3 Transfection efficiency -- 3.5.5 Problems in the application of RNAi technology -- 3.5.5.1 Immune response -- 3.5.5.2 Off-target effect -- 3.5.5.3 Saturation phenomenon -- 3.5.6 The application of RNAi -- 3.5.6.1 Gene function research -- 3.5.6.2 Gene therapy for genetic disease -- 3.5.6.3 The improvement of livestock breed -- 3.6 Transgenic technology of viral vectors -- 3.6.1 Lentiviral vectors -- 3.6.1.1 The structure and source of lentiviral vectors -- 3.6.1.2 The construction of lentiviral vectors -- 3.6.1.3 Advantages and disadvantages of lentiviral vectors -- 3.6.1.4 Applications of lentiviral vectors -- 3.6.2 Adenoviral vectors -- 3.6.2.1 The structure and classification of adenovirus -- 3.6.2.2 The advantages and disadvantages of adenoviral vectors -- 3.6.2.3 Applications of adenovirus vectors -- 3.6.2.3.1 Applications in vaccine research -- 3.6.2.3.2 Gene therapy -- 3.6.3 Adeno-associated virus vector. | |
| 520 | |a Livestock Genome Editing Tools introduces applications and improvements to a series of new genome editing techniques in livestock, such as pigs, cattle, and sheep.These tools include zinc finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN), and the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9). | ||
| 650 | 0 | |a Livestock |x Genetic engineering. | |
| 650 | 7 | |a Livestock |x Genetic engineering |2 fast |0 (OCoLC)fst01000777 | |
| 700 | 1 | |a Li, Kui. | |
| 776 | 0 | 8 | |i Print version: |z 012819099X |z 9780128190999 |w (OCoLC)1412624148 |
| 856 | 4 | 0 | |3 ScienceDirect |u https://www.sciencedirect.com/science/book/9780128190999 |