Livestock genome editing tools /

Livestock Genome Editing Tools introduces applications and improvements to a series of new genome editing techniques in livestock, such as pigs, cattle, and sheep.These tools include zinc finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN), and the clustered regularly int...

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Xehetasun bibliografikoak
Beste egile batzuk:	Li, Kui
Formatua:	eBook
Hizkuntza:	ingelesa
Argitaratua:	London : Academic Press, 2024.
Gaiak:	Livestock > Genetic engineering. Livestock > Genetic engineering
Sarrera elektronikoa:	ScienceDirect
Etiketak:	Etiketa erantsi Etiketarik gabe, Izan zaitez lehena erregistro honi etiketa jartzen!

Aurkibidea:

2.3.8.1 Applications of CRISPR/Cas9 genome editing
2.3.9 Applications of gene expression regulation
2.3.10 Prospects
2.4 Base editing
2.4.1 DNA editing technologies
2.4.1.1 Cytosine base editor technology
2.4.1.2 Adenine-based editor technology
2.4.1.3 RNA editing technology
2.4.1.4 Applications of single-base editing technology
2.4.2 Summary and prospects of single-base editing technology
References
3 Associated technologies for genome editing
3.1 Pronuclear microinjection
3.1.1 Generation of genetically engineered pigs using pronuclear microinjection technology
3.1.1.1 Collection and culture of pronuclear-stage embryos of pigs
3.1.1.1.1 Collection of pronuclear-stage embryos of pigs fertilized under in vivo conditions
Preparation of donor sows
Superovulation and fertilization of donor sows
Recovery of pronuclear-stage embryos
Selection of pronuclear-stage embryos
3.1.1.1.2 In vitro maturation of porcine oocytes and production of pronuclear-stage embryos fertilized under in vitro condi...
In vitro maturation of porcine oocytes
Treatment of mature porcine oocytes
Pretreatment of porcine sperm and in vitro fertilization
3.1.1.1.3 In vitro maturation of porcine oocytes and production of pronuclear-stage embryos by intracytoplasmic sperm injection
Pretreatment of sperm
Preparation of the intracytoplasmic sperm injection operation dish
Intracytoplasmic sperm injection process
3.1.1.2 Production and purification of the target gene fragments
3.1.1.3 Microinjection of zygotes
3.1.1.4 Preparation of recipient sows
3.1.1.5 Embryo transfer
3.1.1.6 Postoperative management of sows and identification of positive individuals
3.1.2 Research status of genetically engineered animal generation by pronuclear microinjection
3.2 Cytoplasmic injection.
3.2.1 Research progress on cytoplasmic injection technology
3.2.2 Mechanism for obtaining transgenes via cytoplasmic injection
3.2.3 Factors affecting cytoplasmic injection
3.2.3.1 Injection technique
3.2.3.2 Size of the zygotes
3.2.3.3 Structure and concentration of exogenous genes
3.2.3.4 Assisted coinjection of other components
3.2.3.5 Injection of a transposon system
3.2.4 Comparison of cytoplasmic and pronuclear microinjections
3.2.4.1 Survival rate of injected embryos
3.2.4.2 Transgenesis efficiency
3.2.4.3 Formation of chimeras
3.2.4.4 Costs
3.2.4.5 Application prospects for CI
3.3 Somatic cell nuclear transfer technology
3.3.1 Introduction of somatic cell nuclear transfer technology
3.3.2 The main methods and processes of somatic cell cloning
3.3.2.1 Preparation of nuclear donor cells
3.3.2.2 Oocyte collection and in vitro maturation
3.3.2.3 Enucleation of recipient oocytes
3.3.2.4 Nuclear transfer of donor cells
3.3.2.5 Fusion and activation of reconstructed embryos
3.3.2.6 In vitro culture and transplantation of reconstructed embryos
3.3.2.7 Care of sows during pregnancy and farrowing
3.3.3 Operation for pig embryo transfer
3.3.3.1 Selection of recipient sows
3.3.3.2 Induction of anesthesia and securing the pig
3.3.3.3 Maintenance of anesthesia and embryo transfer
3.3.3.4 Suturing the incision
3.3.3.5 Care of newborn piglets
3.3.3.5.1 Prevention of the birth of disadvantaged piglets
3.3.3.5.2 Attending and assisting during farrowing
3.3.3.5.3 First aid for dying piglets after birth
3.3.3.5.4 Colostrum intake of newborn piglets
3.3.3.5.5 Crossfostering of newborn piglets
3.3.3.5.6 Environmental requirements for newborn piglets
3.3.3.5.7 Timely iron supplementation for newborn piglets
3.3.3.5.8 Vaccination for piglets.
3.3.4 Present problems in somatic cell cloning technology
3.3.4.1 Low cloning efficiency and low survival rate of offspring
3.3.4.2 The abnormal development of the placenta
3.3.4.3 The source of mitochondria
3.3.4.4 The abnormal process of reprogramming
3.3.5 The prospects of somatic cell cloning technology
3.4 Electroporation
3.4.1 Model and mechanism of electroporation
3.4.2 Application of electroporation in vivo
3.4.3 Factors affecting the efficiency of electroporation
3.4.4 The problems and prospects of electroporation technology
3.5 RNA interference technology
3.5.1 The discovery of RNAi
3.5.2 The mechanism of RNAi
3.5.2.1 Initiation stage
3.5.2.2 Maintenance stage
3.5.2.3 Signal amplification stage
3.5.3 The biological characteristics of RNAi
3.5.4 Factors affecting the efficiency of RNAi
3.5.4.1 The construction of RNAi
3.5.4.2 The modification of RNAi sequences
3.5.4.3 Transfection efficiency
3.5.5 Problems in the application of RNAi technology
3.5.5.1 Immune response
3.5.5.2 Off-target effect
3.5.5.3 Saturation phenomenon
3.5.6 The application of RNAi
3.5.6.1 Gene function research
3.5.6.2 Gene therapy for genetic disease
3.5.6.3 The improvement of livestock breed
3.6 Transgenic technology of viral vectors
3.6.1 Lentiviral vectors
3.6.1.1 The structure and source of lentiviral vectors
3.6.1.2 The construction of lentiviral vectors
3.6.1.3 Advantages and disadvantages of lentiviral vectors
3.6.1.4 Applications of lentiviral vectors
3.6.2 Adenoviral vectors
3.6.2.1 The structure and classification of adenovirus
3.6.2.2 The advantages and disadvantages of adenoviral vectors
3.6.2.3 Applications of adenovirus vectors
3.6.2.3.1 Applications in vaccine research
3.6.2.3.2 Gene therapy
3.6.3 Adeno-associated virus vector.

Livestock genome editing tools /

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